Comparison of Heteropterys aphrodisiaca extraction methods with HPLC

The Heteropterys aphrodisiaca (Ha) root was extracted water, water with heating at 95C for 20min, and 1:1 ethanol-water at 93.33mg/mL. All mixes were sonicated for 2min. The samples were centrifuged at 21130rcf for 8min and the supernatant was analyzed with HPLC-PDA.

HPLC conditions

Column: Phenomenex Luna 5u C18(2) 100A, 250x4.60mm 5micron

Guard column: Phenomenex KrudKatcher Disposable Pre-Column C₁₈, 5mm, 4.6x20 mm

Injection volume: 25uL

Detection: 254nm

Flow rate: 1mL/min

Gradient condition:

Time (min)	Flow (mL/min)	1% acetic acid in water (%)	Acetonitrile (%)
0	1.00	95	5
2	1.00	95	5
33	1.00	5	95
35	1.00	5	95
36	1.00	95	5
46	1.00	95	5
47	0.00	95	5

Heteropterys aphrodisiaca HPLC chromatograms detected at 254nm forextracts with water, water at 95C for 20min, and 1:1 ethanol-water. A 0-35min, B 8-18min.

 A

 

B

The areas of the major peaks between 8-18min of both water extracts were compared to understand the effect of each treatment on the extraction efficiency. All of the major peak areas increased except the peak at 14.61min increased indicating that the extraction was more effective; the decrease in the component at 14.61min was probably due to thermal degradation.